This research focuses on the moderation effects of Hofstede's cultural dimension that influence the relation between ambidextrous leadership and export performance in Born Global Firms in two countries with different cultural characteristics, Vietnam and Colombia. We collected the data from 800 exporting firms in two countries, Colombia and Vietnam, that are characterised by their having begun international operations in the first three years of being Born Global. The results demonstrate that ambidextrous leadership has a positive impact on export performance. Additionally, the dimensions of uncertainty avoidance and indulgence moderate the relation between ambidextrous leadership and export performance, while long-term orientation does not affect the relationship.